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Image Search Results
Journal: Frontiers in oncology
Article Title: Cordycepin Inhibits Triple-Negative Breast Cancer Cell Migration and Invasion by Regulating EMT-TFs SLUG, TWIST1, SNAIL1, and ZEB1.
doi: 10.3389/fonc.2022.898583
Figure Lengend Snippet: FIGURE 4 | Effects of cordycepin on the TWIST1 or SLUG reporter activity. (A) Luciferase reporter assay showed that CD decreased the TWIST1 reporter activity in Hela cells, as the relative light unit (RLU) was decreased with the increase in CD dosage. (B) Luciferase reporter assay showed that CD reduces the SLUG reporter activity in Hela cells, as the Relative Light Units (RLU) were reduced with the increase of CD dosage. *P < 0.05, **P < 0.01. (C, D) The levels of mRNA for TWIST1, SLUG, SNAIL1, and ZEB1 in 4T1 or BT549. 4T1 or BT549 cells were treated with 10 µM of CD for 24 h, total RNA was extracted, and quantitative RT-PCR analysis was performed. (E) The levels of mRNA for TWIST1, SLUG, SNAIL1, and ZEB1 in BT549 were treated with the indicated concentration of CD. BT549 cells were treated with 0, 5, 10, and 20 µM of CD for 24 h, total RNA was extracted, and semiquantitative RT-PCR and quantitative RT-PCR analyses were performed.
Article Snippet:
Techniques: Activity Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Concentration Assay, Reverse Transcription Polymerase Chain Reaction